Douglas Walton
Darnley
Mary Gordon
Nurse
Katharine Hepburn
Mary Stuart
Alan Mowbray
Throckmorton
John Carradine
Rizzio
Donald Crisp
Huntly
Fredric March
Bothwell
Moroni Olsen
John Knox
Alec Craig
Donal
Halliwell Hobbes
Man (uncredited)
David Torrence
Lindsay
Robert Warwick
Sir Francis Knollys
Nigel De Brulier
Judge
Gavin Muir
Leicester
Ralph Forbes
Randolph
Frieda Inescort
Mary Beaton
Ian Keith
Moray
Robert Barrat
Morton
Monte Blue
Messenger
Molly Lamont
Mary Livingstone
Anita Colby
Mary Fleming
Jean Fenwick
Mary Seton
Florence Eldridge
Elizabeth Tudor