Carrie-Anne Moss
Trinity (voice)
Keanu Reeves
Thomas "Neo" Anderson (voice)
Tara Strong
Crew Woman ("Final Flight of Osiris") / Nurse ("World Record") / Misha ("Beyond")
Jill Talley
Mother ("The Second Renaissance Part I & II")
James Arnold Taylor
Additional Voices ("The Second Renaissance Part I & II") / Ash ("A Detective Story") / Raul ("Matriculated")
Pamela Adlon
Jue ("Final Flight of the Osiris") / Manabu ("Beyond") (voice)
Jack Fletcher
Sandro ("Matriculated")
Kevin Michael Richardson
Thadeus ("Final Flight of Osiris") / Cop ("Kid's Story") / Agent #2 ("World Record")
Victor Williams
Dan ("World Record")
Dwight Schultz
Additional Voices ("The Second Renaissance Part I & II") / Monaka ("Matriculated")
Matt McKenzie
Agent #1 ("World Record") / Agent ("Beyond") / Agent ("A Detective Story")
John DiMaggio
Crew Man ("Final Flight of the Osiris") / Kaiser ("Program") (voice)
Phil LaMarr
Duo ("Program")
Tress MacNeille
Housewife ("Beyond") / Kenny ("Beyond")
Akio Otsuka
Thadeus (voice)
Hedy Burress
Cis ("Program") / Yoko ("Beyond")
Olivia d'Abo
Rox ("Matriculated")
Clayton Watson
The Kid (voice)
John DeMita
Teacher ("Kid's Story")
Kath Soucie
Pudgy ("Beyond") / Masa ("Beyond") / Sara ("Beyond")
Debi Derryberry
Kid ("The Second Renaissance Part I & II")
John Wesley
Dan's Dad ("World Record")
Tom Kenny
Operator ("Final Flight of Osiris")
Alex Fernandez
Tom ("World Record")
Melinda Clarke
Alexa ("Matriculated") (voice)
Rick Gomez
Pilot ("Final Flight of Osiris")
Allison Smith
Reporter ("World Record")
Rodney Saulsberry
Chyron ("Matriculated")
Terrence 'T.C.' Carson
Clarence ("A Detective Story")
Bette Ford
Old Woman ("Final Flight of Osiris")