Billy Barty
Googy Gopher / Orville Pelican
Lou Wagner
Don Messick
Freddy the Flute / Googy Gopher / Orson Vulture (voice)
Felix Silla
Polkadotted Horse
Johnny Silver
Dr. Blinky / Ludicrous Lion
Joan Gerber
Dowager Tree / Granddaughter Clock (voice)
Angelo Rossitto
Seymore Spider / Clang
Martha Raye
Boss Witch
Billie Hayes
Witchiepoo
Jack Wild
Jimmy
Allan Melvin
H. R. Pufnstuf / Henrich Rat / Living Island Boat / Orville Pelican / Polka-Dotted Horse / Stupid Bat / West Wind (voice) (as Al Melvin)
Cass Elliot
Witch Hazel (as Mama Cass)
Sharon Baird
Shirley Pufnstuf
Jane Dulo
Miss Flick